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Proceedings Paper

Volumetric imaging of oral epithelial neoplasia by MPM-SHGM: epithelial connective tissue interface (Conference Presentation)
Author(s): Rahul Pal; Jinping Yang; Suimin Qiu; Vicente Resto; Susan McCammon; Gracie Vargas

Paper Abstract

The majority of oral cancers are comprised of oral squamous cell carcinoma in which neoplastic epithelial cells invade across the epithelial connective tissue interface (ECTI). Invasion is preceded by a multi-component process including epithelial hyperproliferation, loss of cell polarity, and remodeling of the extracellular matrix. Multiphoton Autofluorescence Microscopy (MPAM) and Second Harmonic Generation Microscopy (SHGM) show promise for revealing indicators of neoplasia. In particular, volumetric imaging by these methods can reveal aspects of the 3D microstructure that are not possible by other methods and which could both further our understanding of neoplastic transformation and be explored for development of diagnostic approaches in this disease having only 55% 5-year survival rate. MPAM-SHG were applied to reveal the 3D structure of the critical ECTI interface that plays an integral part toward invasion. Epithelial dysplasia was induced in an established hamster model. MPAM-SHGM was applied to lesion sites, using 780 nm excitation (450-600nm emission) for autofluroescence of cellular and extracellular components; 840 nm using 420 nm bandpass filter for SHG. The ECTI surface was identified as the interface at which SHG signal began following the epithelium and was modeled as a 3D surface using Matlab. ECTI surface area and cell features at sites of epithelial expansion where ECTI was altered were measured; Imaged sites were biopsied and processed for histology. ROC analysis using ECTI image metrics indicated the ability to delineate normal from neoplasia with high sensitivity and specificity and it is noteworthy that inflammation did not significantly alter diagnostic potential of MPAM-SHGM .

Paper Details

Date Published: 27 April 2016
PDF: 1 pages
Proc. SPIE 9712, Multiphoton Microscopy in the Biomedical Sciences XVI, 971216 (27 April 2016); doi: 10.1117/12.2213942
Show Author Affiliations
Rahul Pal, The Univ. of Texas Medical Branch (United States)
Jinping Yang, The Univ. of Texas Medical Branch (United States)
Suimin Qiu, The Univ. of Texas Medical Branch (United States)
Vicente Resto, The Univ. of Texas Medical Branch (United States)
Susan McCammon, The Univ. of Texas Medical Branch (United States)
Gracie Vargas, The Univ. of Texas Medical Branch (United States)

Published in SPIE Proceedings Vol. 9712:
Multiphoton Microscopy in the Biomedical Sciences XVI
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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