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Proceedings Paper

Large area 3-D optical coherence tomography imaging of lumpectomy specimens for radiation treatment planning
Author(s): Cuihuan Wang; Leonard Kim; Nicola Barnard; Atif Khan; Mark C. Pierce
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Paper Abstract

Our long term goal is to develop a high-resolution imaging method for comprehensive assessment of tissue removed during lumpectomy procedures. By identifying regions of high-grade disease within the excised specimen, we aim to develop patient-specific post-operative radiation treatment regimens. We have assembled a benchtop spectral-domain optical coherence tomography (SD-OCT) system with 1320 nm center wavelength. Automated beam scanning enables “sub-volumes” spanning 5 mm x 5 mm x 2 mm (500 A-lines x 500 B-scans x 2 mm in depth) to be collected in under 15 seconds. A motorized sample positioning stage enables multiple sub-volumes to be acquired across an entire tissue specimen. Sub-volumes are rendered from individual B-scans in 3D Slicer software and en face (XY) images are extracted at specific depths. These images are then tiled together using MosaicJ software to produce a large area en face view (up to 40 mm x 25 mm). After OCT imaging, specimens were sectioned and stained with HE, allowing comparison between OCT image features and disease markers on histopathology. This manuscript describes the technical aspects of image acquisition and reconstruction, and reports initial qualitative comparison between large area en face OCT images and HE stained tissue sections. Future goals include developing image reconstruction algorithms for mapping an entire sample, and registering OCT image volumes with clinical CT and MRI images for post-operative treatment planning.

Paper Details

Date Published: 29 February 2016
PDF: 5 pages
Proc. SPIE 9689, Photonic Therapeutics and Diagnostics XII, 968946 (29 February 2016); doi: 10.1117/12.2213404
Show Author Affiliations
Cuihuan Wang, Rutgers, The State Univ. of New Jersey (United States)
Leonard Kim, MD Anderson Cancer Ctr. at Cooper (United States)
Nicola Barnard, Robert Wood Johnson Medical School (United States)
Atif Khan, Rutgers Cancer Institute of New Jersey (United States)
Mark C. Pierce, Rutgers, The State Univ. of New Jersey (United States)

Published in SPIE Proceedings Vol. 9689:
Photonic Therapeutics and Diagnostics XII
Hyun Wook Kang; Guillermo J. Tearney M.D.; Melissa C. Skala; Bernard Choi; Andreas Mandelis; Brian J. F. Wong M.D.; Justus F. Ilgner M.D.; Nikiforos Kollias; Paul J. Campagnola; Kenton W. Gregory M.D.; Laura Marcu; Haishan Zeng, Editor(s)

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