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Proceedings Paper

Intraoperative brain hemodynamic response assessment with real-time hyperspectral optical imaging (Conference Presentation)
Author(s): Audrey Laurence; Julien Pichette; Leticia M. Angulo-Rodríguez; Catherine Saint Pierre M.D.; Frédéric Lesage; Alain Bouthillier M.D.; Dang Khoa Nguyen; Frédéric Leblond

Paper Abstract

Following normal neuronal activity, there is an increase in cerebral blood flow and cerebral blood volume to provide oxygenated hemoglobin to active neurons. For abnormal activity such as epileptiform discharges, this hemodynamic response may be inadequate to meet the high metabolic demands. To verify this hypothesis, we developed a novel hyperspectral imaging system able to monitor real-time cortical hemodynamic changes during brain surgery. The imaging system is directly integrated into a surgical microscope, using the white-light source for illumination. A snapshot hyperspectral camera is used for detection (4x4 mosaic filter array detecting 16 wavelengths simultaneously). We present calibration experiments where phantoms made of intralipid and food dyes were imaged. Relative concentrations of three dyes were recovered at a video rate of 30 frames per second. We also present hyperspectral recordings during brain surgery of epileptic patients with concurrent electrocorticography recordings. Relative concentration maps of oxygenated and deoxygenated hemoglobin were extracted from the data, allowing real-time studies of hemodynamic changes with a good spatial resolution. Finally, we present preliminary results on phantoms obtained with an integrated spatial frequency domain imaging system to recover tissue optical properties. This additional module, used together with the hyperspectral imaging system, will allow quantification of hemoglobin concentrations maps. Our hyperspectral imaging system offers a new tool to analyze hemodynamic changes, especially in the case of epileptiform discharges. It also offers an opportunity to study brain connectivity by analyzing correlations between hemodynamic responses of different tissue regions.

Paper Details

Date Published: 26 April 2016
PDF: 1 pages
Proc. SPIE 9690, Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation, 96900E (26 April 2016); doi: 10.1117/12.2213384
Show Author Affiliations
Audrey Laurence, Ecole Polytechnique de Montréal (Canada)
Julien Pichette, Ecole Polytechnique de Montréal (Canada)
Leticia M. Angulo-Rodríguez, Ecole Polytechnique de Montréal (Canada)
Catherine Saint Pierre M.D., Ecole Polytechnique de Montréal (Canada)
Frédéric Lesage, Ecole Polytechnique de Montréal (Canada)
Alain Bouthillier M.D., Univ. de Montréal (Canada)
Dang Khoa Nguyen, Ctr. Hospitalier de l'Univ. de Montréal (Canada)
Frédéric Leblond, Ecole Polytechnique de Montréal (Canada)

Published in SPIE Proceedings Vol. 9690:
Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation
Steen J. Madsen; E. Duco Jansen; Samarendra K. Mohanty; Nitish V. Thakor; Qingming Luo; Victor X. D. Yang, Editor(s)

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