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Proceedings Paper

NIR and MR imaging supported hydrogel based delivery system for anti-TNF alpha probiotic therapy of IBD
Author(s): Jelena M. Janjic; Ales Berlec; Christina Bagia; Lu S. Liu; Irenej Jeric; Michael Gach; Bratislav M. Janjic; Borut Strukelj
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Paper Abstract

Current treatment of inflammatory bowel disease (IBD) is largely symptomatic and consists of anti-inflammatory agents, immune-suppressives or antibiotics, whereby local luminal action is preferred to minimize systemic side-effects. Recently, anti-TNFα therapy has shown considerable success and is now being routinely used. Here we present a novel approach of using perfluorocarbon (PFC) nanoemulsion containing hydrogels (nanoemulgels) as imaging supported delivery systems for anti-TNF alpha probiotic delivery in IBD. To further facilitate image-guided therapy a food-grade lactic acid bacterium Lactococcus lactis capable of TNFα-binding was engineered to incorporate infrared fluorescent protein (IRFP). This modified bacteria was then incorporated into novel PFC nanoemulgels. The nanoemulgels presented here are designed to deliver locally anti-TNFα probiotic in the lower colon and rectum and provide dual imaging signature of gel delivery (MRI) across the rectum and lower colon and bacteria release (NIR). NIR imaging data in vitro demonstrates high IRFP expressing and TNFα-binding bacteria loading in the hydrogel and complete release in 3 hours. Stability tests indicate that gels remain stable for at least 14 days showing no significant change in droplet size, zeta potential and pH. Flow cytometry analyses demonstrate the NIRF expressing bacteria L. lactis binds TNFα in vitro upon release from the gels. Magnetic resonance and near-infrared imaging in vitro demonstrates homogeneity of hydrogels and the imaging capacity of the overall formulation.

Paper Details

Date Published: 22 April 2016
PDF: 10 pages
Proc. SPIE 9723, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VIII, 972309 (22 April 2016); doi: 10.1117/12.2213376
Show Author Affiliations
Jelena M. Janjic, Duquesne Univ. (United States)
McGowan Institute for Regenerative Medicine (United States)
Ales Berlec, Jožef Stefan Institute (Slovenia)
Christina Bagia, Duquesne Univ. (United States)
Lu S. Liu, Duquesne Univ. (United States)
Irenej Jeric, Duquesne Univ. (United States)
Michael Gach, Washington Univ. School of Medicine in St. Louis (United States)
Bratislav M. Janjic, Univ. of Pittsburgh Cancer Institute (United States)
Borut Strukelj, Jožef Stefan Institute (Slovenia)
Univ. of Ljubljana (Slovenia)

Published in SPIE Proceedings Vol. 9723:
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VIII
Samuel Achilefu; Ramesh Raghavachari, Editor(s)

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