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Proceedings Paper

All-in-one detector of circulating mRNA based on a smartphone
Author(s): Vratislav Cmiel; Jaromir Gumulec; Ondrej Svoboda; Martina Raudenska; Kristyna Hudcova; Jiri Sekora; Jaroslav Balogh; Michal Masarik; Ivo Provaznik
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Paper Abstract

Metallothionein is significantly elevated in various tumors, notably in prostate cancer on both mRNA and protein level. We demonstrated a strong predictive potential of free circulating metallothionein 2A isoform mRNA for patients with this cancer. Circulating mRNA detection relies on expensive equipment and requires high level of expertise. In this work we developed compact "all-in-one" laboratory system which replace microvolume spectrophotometer, thermocycler and realtime PCR machines. We managed to design and construct a microprocessor controlled heating/cooling chamber that ensures required temperature gradient. The chamber includes implemented optical system to enable fluorescence excitation and fluorescence analysis using a smart-phone.

Paper Details

Date Published: 4 March 2016
PDF: 6 pages
Proc. SPIE 9715, Optical Diagnostics and Sensing XVI: Toward Point-of-Care Diagnostics, 971509 (4 March 2016); doi: 10.1117/12.2213253
Show Author Affiliations
Vratislav Cmiel, Brno Univ. of Technology (Czech Republic)
Jaromir Gumulec, Masaryk Univ. (Czech Republic)
Ondrej Svoboda, Brno Univ. of Technology (Czech Republic)
Martina Raudenska, Masaryk Univ. (Czech Republic)
Kristyna Hudcova, Masaryk Univ. (Czech Republic)
Jiri Sekora, Brno Univ. of Technology (Czech Republic)
Jaroslav Balogh, Brno Univ. of Technology (Czech Republic)
Michal Masarik, Masaryk Univ. (Czech Republic)
Ivo Provaznik, Brno Univ. of Technology (Czech Republic)

Published in SPIE Proceedings Vol. 9715:
Optical Diagnostics and Sensing XVI: Toward Point-of-Care Diagnostics
Gerard L. Coté, Editor(s)

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