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Proceedings Paper

Monitoring longitudinal changes in irradiated head and neck cancer xenografts using diffuse reflectance spectroscopy
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Paper Abstract

Radiation therapy is often used as the preferred clinical treatment for control of localized head and neck cancer. However, during the course of treatment (6-8 weeks), feedback about functional and/or physiological changes within impacted tissue are not obtained, given the onerous financial and/or logistical burdens of scheduling MRI, PET or CT scans. Diffuse optical sensing is well suited to address this problem since the instrumentation can be made low-cost and portable while still being able to non-invasively provide information about vascular oxygenation in vivo. Here we report results from studies that employed an optical fiber-based portable diffuse reflectance spectroscopy (DRS) system to longitudinally monitor changes in tumor vasculature within two head and neck cancer cell lines (SCC-15 and FaDu) xenografted in the flanks of nude mice, in two separate experiments. Once the tumor volumes were 100mm3, 67% of animals received localized (electron beam) radiation therapy in five fractions (8Gy/day, for 5 days) while 33% of the animals served as controls. DRS measurements were obtained from each animal on each day of treatment and then for two weeks post-treatment. Reflectance spectra were parametrized to extract total hemoglobin concentration and blood oxygen-saturation and the resulting time-trends of optical parameters appear to be dissimilar for the two cell-lines. These findings are also compared to previous animal experiments (using the FaDu line) that were irradiated using a photon beam radiotherapy protocol. These results and implications for the use of fiber-based DRS measurements made at local (irradiated) tumor site as a basis for identifying early radiotherapy-response are presented and discussed.

Paper Details

Date Published: 29 February 2016
PDF: 8 pages
Proc. SPIE 9689, Photonic Therapeutics and Diagnostics XII, 96891W (29 February 2016); doi: 10.1117/12.2213192
Show Author Affiliations
Karthik Vishwanath, Miami Univ. (United States)
Shudong Jiang, Thayer School of Engineering at Dartmouth (United States)
Jason R. Gunn, Thayer School of Engineering at Dartmouth (United States)
Kayla Marra, Thayer School of Engineering at Dartmouth (United States)
Jacqueline Marie Andreozzi, Thayer School of Engineering at Dartmouth (United States)
Brian W. Pogue, Thayer School of Engineering at Dartmouth (United States)

Published in SPIE Proceedings Vol. 9689:
Photonic Therapeutics and Diagnostics XII
Hyun Wook Kang; Guillermo J. Tearney M.D.; Melissa C. Skala; Bernard Choi; Andreas Mandelis; Brian J. F. Wong M.D.; Justus F. Ilgner M.D.; Nikiforos Kollias; Paul J. Campagnola; Kenton W. Gregory M.D.; Laura Marcu; Haishan Zeng, Editor(s)

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