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Proceedings Paper

Photothermal inactivation of bacteria on plasmonic nanostructures
Author(s): Greggy M. Santos; Felipe Ibañez de Santi Ferrara; Fusheng Zhao; Debora F. Rodrigues; Wei-Chuan Shih
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Paper Abstract

Hospital-acquired bacterial infections are frequently associated with the pathogenic biofilms on surfaces of devices and instruments used in medical procedures. The utilization of thermal plasmonic agents is an innovative approach for sterilizing hospital equipment and for in vivo therapeutic treatment of bacterial infection. A photothermal inactivation technique via array of nanoporous gold disks (NPGDs) has been developed by irradiating near infrared (NIR) light onto deposited bacterial cells (Escherichia coli, Bacillus subtilis, Exiguobacterium AT1B) on the surface of metal nanostructure. The physical and photothermal properties of the NPGD substrate were investigated using topographical scanning electron microscopy (SEM) and thermographic infrared imaging. Bacterial viability studies on NPGD substrates irradiated with and without NIR light were evaluated using a fluorescence-based two-component stain assay. The results show that the heat generated from the NPGD substrate promotes high cell death counts (~100%) at short exposure durations (<25 s) even for thermally-resistant bacterial strains. The photothermal effects on NPGD substrate can lead to point-of-care applications.

Paper Details

Date Published: 22 April 2016
PDF: 6 pages
Proc. SPIE 9724, Plasmonics in Biology and Medicine XIII, 97240D (22 April 2016); doi: 10.1117/12.2213191
Show Author Affiliations
Greggy M. Santos, Univ. of Houston (United States)
Felipe Ibañez de Santi Ferrara, Univ. of Houston (United States)
Ministry of Education of Brazil (Brazil)
Fusheng Zhao, Univ. of Houston (United States)
Debora F. Rodrigues, Univ. of Houston (United States)
Wei-Chuan Shih, Univ. of Houston (United States)

Published in SPIE Proceedings Vol. 9724:
Plasmonics in Biology and Medicine XIII
Tuan Vo-Dinh; Joseph R. Lakowicz, Editor(s)

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