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Proceedings Paper

Development of a temporal multiplexed 3D beam-scanning Lissajous trajectory microscope for rapid multimodal volumetric imaging
Author(s): Justin A. Newman; Shane Z. Sullivan; Janny Dinh; Sreya Sarkar; Garth J. Simpson
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Paper Abstract

A beam-scanning microscope is described based on a temporally multiplexed Lissajous trajectory for achieving 1 kHz frame rate 3D imaging. The microscope utilizes two fast-scan resonant mirrors to direct the optical beam on a circuitous, Lissajous trajectory through the field of view. Acquisition of two simultaneous focal planes is achieved by implementation of an optical delay line, producing a second incident beam at a different focal plane relative to the initial incident beam. High frame rates are achieved by separating the full time-domain data into shorter sub-trajectories resulting in undersampling of the field of view. A model-based image reconstruction (MBIR) 3D in-painting algorithm is utilized for interpolating the missing data to recover full images. The MBIR algorithm uses a maximum a posteriori estimation with a generalized Gaussian Markov random field prior model for image interpolation. Because images are acquired using photomultiplier tubes or photodiodes, parallelization for multi-channel imaging is straightforward.

Preliminary results obtained using a Lissajous trajectory beam-scanning approach coupled with temporal multiplexing by the implementation of an optical delay line demonstrate the ability to acquire 2 distinct focal planes simultaneously at frame rates >450 Hz for full 512 × 512 images. The use of multi-channel data acquisition cards allows for simultaneous multimodal image acquisition with perfect image registry between all imaging modalities. Also discussed here is the implementation of Lissajous trajectory beam-scanning on commercially available microscope hardware.

Paper Details

Date Published: 9 March 2016
PDF: 6 pages
Proc. SPIE 9713, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII, 971312 (9 March 2016); doi: 10.1117/12.2212991
Show Author Affiliations
Justin A. Newman, Purdue Univ. (United States)
Shane Z. Sullivan, Purdue Univ. (United States)
Janny Dinh, Purdue Univ. (United States)
Sreya Sarkar, Purdue Univ. (United States)
Garth J. Simpson, Purdue Univ. (United States)

Published in SPIE Proceedings Vol. 9713:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII
Thomas G. Brown; Carol J. Cogswell; Tony Wilson, Editor(s)

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