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Proceedings Paper

FT-IR spectroscopy characterization of schwannoma: a case study
Author(s): Isabelle Ferreira; Lazaro P. M. Neto; Maurilio José das Chagas; Luís Felipe C. S. Carvalho; Laurita dos Santos; Marcelo Ribas; Vinicius Loddi; Airton A. Martin
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Paper Abstract

Schwannoma are rare benign neural neoplasia. The clinical diagnosis could be improved if novel optical techniques are performed. Among these techniques, FT-IR is one of the currently techniques which has been applied for samples discrimination using biochemical information with minimum sample preparation. In this work, we report a case of a schwannoma in the cervical region. A histological examination described a benign process. An immunohistochemically examination demonstrated positivity to anti-S100 protein antibody, indicating a diagnosis of schwannoma. The aim of this analysis was to characterize FT-IR spectrum of the neoplastic and normal tissue in the fingerprint (1000-1800 cm-1) and high wavenumber region (2800-3600 cm-1). The IR spectra were collect from tumor tissue and normal nerve samples by a FT-IR spectrophotometer (Spotlight Perkin Elmer 400, USA) with 64 scans, and resolution of 4 cm−1. A total of twenty spectra were recorded (10 from schwannoma and 10 from nerve). Multivariate Analysis was used to classify the data. Through average and standard deviation analysis we observed that the main spectral change occurs at ≈1600 cm-1 (amide I) and ≈1400 cm-1 (amide III) in the fingerprint region, and in CH2/CH3 protein-lipids and OH-water vibrations for the high wavenumber region. In conclusion, FT-IR could be used as a technique for schwannoma analysis helping to establish specific diagnostic.

Paper Details

Date Published: 7 March 2016
PDF: 4 pages
Proc. SPIE 9704, Biomedical Vibrational Spectroscopy 2016: Advances in Research and Industry, 97040Z (7 March 2016); doi: 10.1117/12.2212557
Show Author Affiliations
Isabelle Ferreira, Univ. do Vale do Paraíba (Brazil)
Lazaro P. M. Neto, Univ. do Vale do Paraíba (Brazil)
Maurilio José das Chagas, Univ. do Vale do Paraíba (Brazil)
Luís Felipe C. S. Carvalho, Univ. do Vale do Paraíba (Brazil)
Laurita dos Santos, Univ. do Vale do Paraíba (Brazil)
Marcelo Ribas, Univ. do Vale do Paraíba (Brazil)
Vinicius Loddi, Univ. do Vale do Paraíba (Brazil)
Airton A. Martin, Univ. do Vale do Paraíba (Brazil)

Published in SPIE Proceedings Vol. 9704:
Biomedical Vibrational Spectroscopy 2016: Advances in Research and Industry
Anita Mahadevan-Jansen; Wolfgang Petrich, Editor(s)

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