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Proceedings Paper

Accurate axial localization by conical diffraction beam shaping generating a dark-helix PSF
Author(s): Clement Fallet; Astrid Lassalle; Maxime Dubois-Delumeau; Gabriel Y. Sirat
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Paper Abstract

We present here a new PSF-shaping technique using biaxial crystals to generate a highly z-dependent distribution in single molecule localization microscopy (SMLM). This distribution features two zeros of intensity that rotate together with defocus. This PSF features similarities to the double-helix introduced by Moerner and Piestun and thus has been dubbed dark-helix since we track zeros of intensity. Preliminary numerical studies based on Cramer-Rao Lower Bound (CRLB) show that this PSF has the potential to obtain up to 20nm localization precision. This PSF can be easily generated by a very simple, monolithic add-on added in front of the detection camera. Additionally, the PSF remains of the approximate size of the Airy PSF, the x-y localization precision is not substantially affected and no trade-off is required. The xy compacity of the PSF also enables theoretically a higher density of emitters than the double-helix which spreads on a larger scale. Limiting factors for SMLM such as loss of photons, complexity and robustness will be discussed and considerations about the practical implementation of such techniques will be given.

Paper Details

Date Published: 1 March 2016
PDF: 6 pages
Proc. SPIE 9714, Single Molecule Spectroscopy and Superresolution Imaging IX, 971414 (1 March 2016); doi: 10.1117/12.2212215
Show Author Affiliations
Clement Fallet, Bioaxial SAS (France)
Astrid Lassalle, Bioaxial SAS (France)
Maxime Dubois-Delumeau, Bioaxial SAS (France)
Gabriel Y. Sirat, Bioaxial SAS (France)

Published in SPIE Proceedings Vol. 9714:
Single Molecule Spectroscopy and Superresolution Imaging IX
Jörg Enderlein; Ingo Gregor; Zygmunt Karol Gryczynski; Rainer Erdmann; Felix Koberling, Editor(s)

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