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Proceedings Paper

Generating 3D depletion distribution in an achromatic single-channel monolithic system
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Paper Abstract

Recent developments have shown that conical diffraction by a biaxial crystal can create a vortex beam for use in 2D STED microscopy. It has been shown that this concept can be extended and also generate the depletion distributions used for 3D STED microscopy. A single beam passes through a biaxial crystal that creates two co-propagating, co-localized beams; the first one is used for lateral depletion, and the other one for axial depletion. The two beams are crossed-polarized and thus do not interfere. We will show that the 3D distribution can be made achromatic, i.e. several depletion wavelengths can travel through a common path and still be shaped into the appropriate pattern by optimizing the geometry of the system. This system enables true one-channel 3D depletion at multiple wavelengths ranging from 580nm to 770nm, thus covering most of the conventional depletion wavelengths currently used. Preliminary results of depletion PSFs will be presented and the advantages and limitations of this system will be discussed as well as the experimental considerations required to successfully obtain the desired PSFs.

Paper Details

Date Published: 1 March 2016
PDF: 7 pages
Proc. SPIE 9714, Single Molecule Spectroscopy and Superresolution Imaging IX, 97140H (1 March 2016); doi: 10.1117/12.2212193
Show Author Affiliations
Clement Fallet, Bioaxial SAS (France)
Arvid Lindberg, Bioaxial SAS (France)
Gabriel Y. Sirat, Bioaxial SAS (France)

Published in SPIE Proceedings Vol. 9714:
Single Molecule Spectroscopy and Superresolution Imaging IX
Jörg Enderlein; Ingo Gregor; Zygmunt Karol Gryczynski; Rainer Erdmann; Felix Koberling, Editor(s)

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