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Proceedings Paper

Multi-modal digital holographic microscopy for wide-field fluorescence and 3D phase imaging
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Paper Abstract

Multi-modal digital holographic microscopy is a combination of epifluorescence microscopy and digital holographic microscopy, the main function of which is to obtain images from fluorescence intensity and quantified phase contrasts, simultaneously. The proposed system is mostly beneficial to biological studies, with the reason that often the studies are depending on fluorescent labeling techniques to detect certain intracellular molecules, while phase information reflecting properties of unstained transparent elements. This paper is presenting our latest researches on applications such as randomly moving micro-fluorescent beads and living cells of Physcomitrella patens. The experiments are succeeded on obtaining a succession of wide-field fluorescent images and holograms from micro-beads, and different depths focusing is realized via numerical reconstruction. Living cells of Physcomitrella patens are recorded in the static manner, the reconstruction distance indicates thickness of cellular structure. These results are implementing practical applications toward many biomedical science researches.

Paper Details

Date Published: 9 March 2016
PDF: 6 pages
Proc. SPIE 9718, Quantitative Phase Imaging II, 971821 (9 March 2016); doi: 10.1117/12.2212086
Show Author Affiliations
Xiangyu Quan, Kobe Univ. (Japan)
Peng Xia, Kobe Univ. (Japan)
Osamu Matoba, Kobe Univ. (Japan)
Koichi Nitta, Kobe Univ. (Japan)
Yasuhiro Awatsuji, Kyoto Institute of Technology (Japan)

Published in SPIE Proceedings Vol. 9718:
Quantitative Phase Imaging II
Gabriel Popescu; YongKeun Park, Editor(s)

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