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Proceedings Paper

Label-free chemical imaging of live Euglena gracilis by high-speed SRS spectral microscopy (Conference Presentation)
Author(s): Yoshifumi Wakisaka; Yuta Suzuki; Kyoya Tokunaga; Misa Hirose; Ryota Domon; Rina Akaho; Mai Kuroshima; Norimichi Tsumura; Tomoyoshi Shimobaba; Osamu Iwata; Kengo Suzuki; Ayaka Nakashima; Keisuke Goda; Yasuyuki Ozeki

Paper Abstract

Microbes, especially microalgae, have recently been of great interest for developing novel biofuels, drugs, and biomaterials. Imaging-based screening of live cells can provide high selectivity and is attractive for efficient bio-production from microalgae. Although conventional cellular screening techniques use cell labeling, labeling of microbes is still under development and can interfere with their cellular functions. Furthermore, since live microbes move and change their shapes rapidly, a high-speed imaging technique is required to suppress motion artifacts. Stimulated Raman scattering (SRS) microscopy allows for label-free and high-speed spectral imaging, which helps us visualize chemical components inside biological cells and tissues. Here we demonstrate high-speed SRS imaging, with temporal resolution of 0.14 seconds, of intracellular distributions of lipid, polysaccharide, and chlorophyll concentrations in rapidly moving Euglena gracilis, a unicellular phytoflagellate. Furthermore, we show that our method allows us to analyze the amount of chemical components inside each living cell. Our results indicate that SRS imaging may be applied to label-free screening of living microbes based on chemical information.

Paper Details

Date Published: 28 June 2016
PDF: 1 pages
Proc. SPIE 9720, High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management, 97200H (28 June 2016); doi: 10.1117/12.2211991
Show Author Affiliations
Yoshifumi Wakisaka, The Univ. of Tokyo (Japan)
Yuta Suzuki, The Univ. of Tokyo (Japan)
Kyoya Tokunaga, The Univ. of Tokyo (Japan)
Misa Hirose, Chiba Univ. (Japan)
Ryota Domon, Chiba Univ. (Japan)
Rina Akaho, Chiba Univ. (Japan)
Mai Kuroshima, Chiba Univ. (Japan)
Norimichi Tsumura, Chiba Univ. (Japan)
Tomoyoshi Shimobaba, Chiba Univ. (Japan)
Osamu Iwata, euglena Co., Ltd (Japan)
Kengo Suzuki, euglena Co., Ltd (Japan)
Ayaka Nakashima, euglena Co., Ltd (Japan)
Keisuke Goda, The Univ. of Tokyo (Japan)
Univ. of California (United States)
Japan Science and Technology Agency (Japan)
Yasuyuki Ozeki, The Univ. of Tokyo (Japan)

Published in SPIE Proceedings Vol. 9720:
High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management
Kevin K. Tsia; Keisuke Goda, Editor(s)

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