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Proceedings Paper

Three-photon imaging of ovarian cancer
Author(s): Jennifer Kehlet Barton; Babak Amirsolaimani; Photini Rice; Kenneth Hatch; Khanh Kieu
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Paper Abstract

Optical imaging methods have the potential to detect ovarian cancer at an early, curable stage. Optical imaging has the disadvantage that high resolution techniques require access to the tissue of interest, but miniature endoscopes that traverse the natural orifice of the reproductive tract, or access the ovaries and fallopian tubes through a small incision in the vagina wall, can provide a minimally-invasive solution. We have imaged both rodent and human ovaries and fallopian tubes with a variety of endoscope-compatible modalities. The recent development of fiber-coupled femtosecond lasers will enable endoscopic multiphoton microscopy (MPM). We demonstrated two- and three-photon excited fluorescence (2PEF, 3PEF), and second- and third-harmonic generation microscopy (SHG, THG) in human ovarian and fallopian tube tissue. A study was undertaken to understand the mechanisms of contrast in these images. Six patients (normal, cystadenoma, and ovarian adenocarcinoma) provided ovarian and fallopian tube biopsies. The tissue was imaged with three-dimensional optical coherence tomography, multiphoton microscopy, and frozen for histological sectioning. Tissue sections were stained with hematoxylin and eosin, Masson’s trichrome, and Sudan black. Approximately 1 μm resolution images were obtained with an excitation source at 1550 nm. 2PEF signal was absent. SHG signal was mainly from collagen. 3PEF and THG signal came from a variety of sources, including a strong signal from fatty connective tissue and red blood cells. Adenocarcinoma was characterized by loss of SHG signal, whereas cystic abnormalities showed strong SHG. There was limited overlap of two- and three- photon signals, suggesting that three-photon imaging can provide additional information for early diagnosis of ovarian cancer.

Paper Details

Date Published: 29 February 2016
PDF: 8 pages
Proc. SPIE 9689, Photonic Therapeutics and Diagnostics XII, 96893P (29 February 2016); doi: 10.1117/12.2211798
Show Author Affiliations
Jennifer Kehlet Barton, The Univ. of Arizona (United States)
College of Optical Sciences, The Univ. of Arizona (United States)
Babak Amirsolaimani, College of Optical Sciences, The Univ. of Arizona (United States)
Photini Rice, The Univ. of Arizona (United States)
Kenneth Hatch, The Univ. of Arizona (United States)
Khanh Kieu, College of Optical Sciences, The Univ. of Arizona (United States)

Published in SPIE Proceedings Vol. 9689:
Photonic Therapeutics and Diagnostics XII
Hyun Wook Kang; Guillermo J. Tearney M.D.; Melissa C. Skala; Bernard Choi; Andreas Mandelis; Brian J. F. Wong M.D.; Justus F. Ilgner M.D.; Nikiforos Kollias; Paul J. Campagnola; Kenton W. Gregory M.D.; Laura Marcu; Haishan Zeng, Editor(s)

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