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Proceedings Paper

Fast method of cross-talk effect reduction in biomedical imaging (Conference Presentation)
Author(s): Maciej Nowakowski; Sylwia M. Kolenderska; Dawid Borycki; Maciej Wojtkowski

Paper Abstract

Optical imaging of biological samples or living tissue structures requires light delivery to a region of interest and then collection of scattered light or fluorescent light in order to reconstruct an image of the object. When the coherent illumination light enters bulky biological object, each of scattering center (single molecule, group of molecules or other sample feature) acts as a secondary light source. As a result, scattered spherical waves from these secondary sources interact with each other, generating cross-talk noise between optical channels (eigenmodes). The cross-talk effect have serious impact on the performance of the imaging systems. In particular it reduces an ability of optical system to transfer high spatial frequencies thereby reducing its resolution. In this work we present a fast method to eliminate all unwanted waves combination, that overlap at image plane, suppressing recovery of high spatial frequencies by using the spatio-temporal optical coherence manipulation (STOC, [1]). In this method a number of phase mask is introduced to illuminating beam by spatial light modulator in a time of single image acquisition. We use a digital mirror device (DMD) in order to rapid cross-talk noise reduction (up to 22kHz modulation frequency) when imaging living biological cells in vivo by using full-field microscopy setup with double pass arrangement. This, to our best knowledge, has never been shown before. [1] D. Borycki, M. Nowakowski, and M. Wojtkowski, Opt. Lett. 38, 4817 (2013).

Paper Details

Date Published: 27 April 2016
PDF: 1 pages
Proc. SPIE 9717, Adaptive Optics and Wavefront Control for Biological Systems II, 97170C (27 April 2016); doi: 10.1117/12.2211624
Show Author Affiliations
Maciej Nowakowski, Nicolaus Copernicus Univ. (Poland)
Sylwia M. Kolenderska, Nicolaus Copernicus Univ. (Poland)
Dawid Borycki, Nicolaus Copernicus Univ. (Poland)
Maciej Wojtkowski, Nicolaus Copernicus Univ. (Poland)

Published in SPIE Proceedings Vol. 9717:
Adaptive Optics and Wavefront Control for Biological Systems II
Thomas G. Bifano; Joel Kubby; Sylvain Gigan, Editor(s)

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