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Proceedings Paper

Quantitative label-free sperm imaging by means of transport of intensity
Author(s): Praveen Kumar Poola; Vimal Prabhu Pandiyan; Varshini Jayaraman; Renu John
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Paper Abstract

Most living cells are optically transparent which makes it difficult to visualize them under bright field microscopy. Use of contrast agents or markers and staining procedures are often followed to observe these cells. However, most of these staining agents are toxic and not applicable for live cell imaging. In the last decade, quantitative phase imaging has become an indispensable tool for morphological characterization of the phase objects without any markers. In this paper, we report noninterferometric quantitative phase imaging of live sperm cells by solving transport of intensity equations with recorded intensity measurements along optical axis on a commercial bright field microscope.

Paper Details

Date Published: 9 March 2016
PDF: 7 pages
Proc. SPIE 9718, Quantitative Phase Imaging II, 97180O (9 March 2016); doi: 10.1117/12.2211608
Show Author Affiliations
Praveen Kumar Poola, Indian Institute of Technology Hyderabad (India)
Vimal Prabhu Pandiyan, Indian Institute of Technology Hyderabad (India)
Varshini Jayaraman, Univ. of Hyderabad (India)
Renu John, Indian Institute of Technology Hyderabad (India)

Published in SPIE Proceedings Vol. 9718:
Quantitative Phase Imaging II
Gabriel Popescu; YongKeun Park, Editor(s)

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