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Proceedings Paper

Prospects for soft x-ray contact microscopy using laser plasmas as an x-ray source
Author(s): Anthony D. Stead; Anton M. Page; Thomas W. Ford
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Paper Abstract

Since its invention, a major concern of those using a microscope has been to improve the resolution without the introduction of artifacts. While light microscopy carrier little risk of the introduction of artifacts, because the preparative techniques are often minimal, the resolution is somewhat limited. The advent of the electron microscope offered greatly improved resolution but since biological specimens require extensive preparation, the possiblity of causing structural damage to the specimen is also increased. The ideal technique for structural studies of biological specimens would enable hydrated material to be examined without any preparation and with a resolution equal to that of electron microscopy. Soft x-ray microscopy certainly enables living material to be examined and while the resolution does not equal that of electron microscopy it exceeds that attainable by light microscopy. This paper briefly reviews the limitations of light and electron microscopy for the biologist the various ways that soft x- rays might be used to image hydrated biological material. Consideration is given to the different sources that have been used for soft x-ray microscopy and the relative merits of laser-plasma sources are discussed.

Paper Details

Date Published: 18 September 1995
PDF: 11 pages
Proc. SPIE 2523, Applications of Laser Plasma Radiation II, (18 September 1995); doi: 10.1117/12.220995
Show Author Affiliations
Anthony D. Stead, Univ. of London (United Kingdom)
Anton M. Page, Univ. of London (United Kingdom)
Thomas W. Ford, Univ. of London (United Kingdom)

Published in SPIE Proceedings Vol. 2523:
Applications of Laser Plasma Radiation II
Martin C. Richardson; George A. Kyrala, Editor(s)

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