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Proceedings Paper

Label-free imaging of developing vasculature in zebrafish with phase variance optical coherence microscopy
Author(s): Yu Chen; Jeff Fingler; Le A. Trinh; Scott E. Fraser
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Paper Abstract

A phase variance optical coherence microscope (pvOCM) has been created to visualize blood flow in the vasculature of zebrafish embryos, without using exogenous labels. The pvOCM imaging system has axial and lateral resolutions of 2 μm in tissue, and imaging depth of more than 100 μm. Imaging of 2–5 days post-fertilization zebrafish embryos identified the detailed structures of somites, spinal cord, gut and notochord based on intensity contrast. Visualization of the blood flow in the aorta, veins and intersegmental vessels was achieved with phase variance contrast. The pvOCM vasculature images were confirmed with corresponding fluorescence microscopy of a zebrafish transgene that labels the vasculature with green fluorescent protein. The pvOCM images also revealed functional information of the blood flow activities that is crucial for the study of vascular development.

Paper Details

Date Published: 9 March 2016
PDF: 7 pages
Proc. SPIE 9716, Optical Methods in Developmental Biology IV, 97160A (9 March 2016); doi: 10.1117/12.2209653
Show Author Affiliations
Yu Chen, Univ. of Southern California (United States)
Jeff Fingler, Varocto, Inc. (United States)
Le A. Trinh, Univ. of Southern California (United States)
Scott E. Fraser, Univ. of Southern California (United States)

Published in SPIE Proceedings Vol. 9716:
Optical Methods in Developmental Biology IV
Andrew M. Rollins; Scott E. Fraser; Michael A. Choma, Editor(s)

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