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Proceedings Paper

Blue light inhibits proliferation of melanoma cells
Author(s): Anja Becker; Elisabeth Distler; Anna Klapczynski; Fabiola Arpino; Natalia Kuch; Katja Simon-Keller; Carsten Sticht; Frank A. van Abeelen; Norbert Gretz; Gerrit Oversluizen
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Paper Abstract

Photobiomodulation with blue light is used for several treatment paradigms such as neonatal jaundice, psoriasis and back pain. However, little is known about possible side effects concerning melanoma cells in the skin. The aim of this study was to assess the safety of blue LED irradiation with respect to proliferation of melanoma cells. For that purpose we used the human malignant melanoma cell line SK-MEL28. Cell proliferation was decreased in blue light irradiated cells where the effect size depended on light irradiation dosage. Furthermore, with a repeated irradiation of the melanoma cells on two consecutive days the effect could be intensified. Fluorescence-activated cell sorting with Annexin V and Propidium iodide labeling did not show a higher number of dead cells after blue light irradiation compared to non-irradiated cells. Gene expression analysis revealed down-regulated genes in pathways connected to anti-inflammatory response, like B cell signaling and phagosome. Most prominent pathways with up-regulation of genes were cytochrome P450, steroid hormone biosynthesis. Furthermore, even though cells showed a decrease in proliferation, genes connected to the cell cycle were up-regulated after 24h. This result is concordant with XTT test 48h after irradiation, where irradiated cells showed the same proliferation as the no light negative control. In summary, proliferation of melanoma cells can be decreased using blue light irradiation. Nevertheless, the gene expression analysis has to be further evaluated and more studies, such as in-vivo experiments, are warranted to further assess the safety of blue light treatment.

Paper Details

Date Published: 8 March 2016
PDF: 10 pages
Proc. SPIE 9695, Mechanisms of Photobiomodulation Therapy XI, 969503 (8 March 2016); doi: 10.1117/12.2209504
Show Author Affiliations
Anja Becker, Ruprecht-Karls-Univ. Heidelberg (Germany)
Elisabeth Distler, Ruprecht-Karls-Univ. Heidelberg (Germany)
Anna Klapczynski, Ruprecht-Karls-Univ. Heidelberg (Germany)
Fabiola Arpino, Ruprecht-Karls-Univ. Heidelberg (Germany)
Natalia Kuch, Ruprecht-Karls-Univ. Heidelberg (Germany)
Katja Simon-Keller, Ruprecht-Karls-Univ. Heidelberg (Germany)
Carsten Sticht, Ruprecht-Karls-Univ. Heidelberg (Germany)
Frank A. van Abeelen, Philips Research (Netherlands)
Norbert Gretz, Ruprecht-Karls-Univ. Heidelberg (Germany)
Gerrit Oversluizen, Philips Research (Netherlands)

Published in SPIE Proceedings Vol. 9695:
Mechanisms of Photobiomodulation Therapy XI
Michael R. Hamblin; James D. Carroll; Praveen Arany, Editor(s)

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