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Proceedings Paper

Quantitative assessment of optical properties in healthy cartilage and repair tissue by optical coherence tomography and histology (Conference Presentation)
Author(s): Sanne M. A. Jansen M.D.; Paul Cernohorsky M.D.; Daniel M. de Bruin; Edwin van der Pol; Cemile D. Savci-Heijink; Simon D. Strackee M.D.; Dirk J. Faber; Ton G. van Leeuwen

Paper Abstract

Quantification of the OCT signal is an important step toward clinical implementation of a diagnostic tool in cartilage imaging. Discrimination of structural cartilage differences in patients with osteoarthritis is critical, yet challenging. This study assesses the variation in the optical attenuation coefficient (μOCT) between healthy cartilage, repair tissue, bone and layers within repair tissue in a controlled setting. OCT and histology was used to assess goat talus articular surfaces in which central osteochondral defects were created. Exact matches of OCT and histology were selected for research. μOCT measurements were taken from healthy cartilage, repair tissue and bone. Measured μOCT in healthy cartilage was higher compared to both repair tissue and bone tissue. Two possible mechanisms for the difference in attenuation were investigated. We studied morphological parameters in terms of nucleus count, nucleus size and inter-nucleus distance. Collagen content in healthy cartilage and repair tissue was assessed using polarization microscopy. Quantitative analysis of the nuclei did not demonstrate a difference in nucleus size and count between healthy cartilage and repair tissue. In healthy cartilage, cells were spaced farther apart and had a lower variation in local nuclear density compared to repair tissue. Polarization microscopy suggested higher collagen content in healthy cartilage compared to repair tissue. μOCT measurements can distinguish between healthy cartilage, repair tissue and bone. Results suggest that cartilage OCT attenuation measurements could be of great impact in clinical diagnostics of osteoarthritis.

Paper Details

Date Published: 27 April 2016
PDF: 1 pages
Proc. SPIE 9689, Photonic Therapeutics and Diagnostics XII, 96894P (27 April 2016); doi: 10.1117/12.2209341
Show Author Affiliations
Sanne M. A. Jansen M.D., Academisch Medisch Centrum (Netherlands)
Paul Cernohorsky M.D., Academisch Medisch Centrum (Netherlands)
Daniel M. de Bruin, Academisch Medisch Centrum (Netherlands)
Edwin van der Pol, Academisch Medisch Centrum (Netherlands)
Cemile D. Savci-Heijink, Academisch Medisch Centrum (Netherlands)
Simon D. Strackee M.D., Academisch Medisch Centrum (Netherlands)
Dirk J. Faber, Academisch Medisch Centrum (Netherlands)
Ton G. van Leeuwen, Academisch Medisch Centrum (Netherlands)

Published in SPIE Proceedings Vol. 9689:
Photonic Therapeutics and Diagnostics XII
Hyun Wook Kang; Guillermo J. Tearney M.D.; Melissa C. Skala; Bernard Choi; Andreas Mandelis; Brian J. F. Wong M.D.; Justus F. Ilgner M.D.; Nikiforos Kollias; Paul J. Campagnola; Kenton W. Gregory M.D.; Laura Marcu; Haishan Zeng, Editor(s)

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