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Proceedings Paper

1300 nm and 890 nm OCT images of oral cancer tissue engineered models and biopsy samples offer complimentary performance (Conference Presentation)
Author(s): Joseph Boadi; Robert A. Byers; Jon Fernandes; Shweta Mittar; Vanessa Hearnden; Zenghai Lu; Sheila MacNeil; Martin Thornhill; Craig Murdoch; Keith D. Hunter; Alasdair McKechnie; Stephen J. Matcher

Paper Abstract

OCT has demonstrated great potential to non-invasively detect oral epithelial cancers, potentially guiding biopsy and surgical resection. On non-ophthalmic tissues the preferred illumination wavelength is 1300 nm. Previous studies on skin have shown that useful image data can also be obtained at shorter wavelengths, with systems at 1060 nm and 820 nm offering reduced depth penetration but higher contrast. Here we apply a similar comparison to tissue engineered models of oral cancer and also to human biopsy samples, generally finding a similar trend. 1300 nm multi-beam OCT (Michelson Diagnostics EX1301) visualises stromal structures and surface keratin more clearly, providing useful image contrast down to around 1 mm. This system was compared with an ultra-high resolution home-built system operating at 890 nm (2.5 micron resolution vs 7.5 micron axial resolution for the EX1301). The UHR system reveals epithelial features more clearly, especially in the DOK pre-invasive cell line model and the biopsy samples. The relative effects of center wavelength vs axial resolution in generating the differential, wavelength-dependent contrast are assessed and the OCT biopsy images are compared with contemporary histology.

Paper Details

Date Published: 27 April 2016
PDF: 1 pages
Proc. SPIE 9689, Photonic Therapeutics and Diagnostics XII, 96891Z (27 April 2016); doi: 10.1117/12.2209310
Show Author Affiliations
Joseph Boadi, The Univ. of Sheffield (United Kingdom)
Robert A. Byers, The Univ. of Sheffield (United Kingdom)
Jon Fernandes, The Univ. of Sheffield (United Kingdom)
Shweta Mittar, The Univ. of Sheffield (United Kingdom)
Vanessa Hearnden, The Univ. of Sheffield (United Kingdom)
Zenghai Lu, The Univ. of Sheffield (United Kingdom)
Sheila MacNeil, The Univ. of Sheffield (United Kingdom)
Martin Thornhill, The Univ. of Sheffield (United Kingdom)
Craig Murdoch, The Univ. of Sheffield (United Kingdom)
Keith D. Hunter, The Univ. of Sheffield (United Kingdom)
Alasdair McKechnie, The Univ. of Sheffield (United Kingdom)
Stephen J. Matcher, The Univ. of Sheffield (United Kingdom)

Published in SPIE Proceedings Vol. 9689:
Photonic Therapeutics and Diagnostics XII
Hyun Wook Kang; Guillermo J. Tearney M.D.; Melissa C. Skala; Bernard Choi; Andreas Mandelis; Brian J. F. Wong M.D.; Justus F. Ilgner M.D.; Nikiforos Kollias; Paul J. Campagnola; Kenton W. Gregory M.D.; Laura Marcu; Haishan Zeng, Editor(s)

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