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Proceedings Paper

A practical optical-resolution photoacoustic microscopy prototype using a 300 mW visible laser diode
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Paper Abstract

Optical-resolution photoacoustic microscopy (OR-PAM) is an emerging technique for microvasculature imaging at high spatial resolution and contrast. In this work, we present a practical visible laser-diode-based OR-PAM (LD-OR-PAM) prototype for vasculature imaging, which has the desirable properties of being portable, low-cost, and label-free. The prototype employs a 300 mW pulsed laser diode in a 3.8 mm diameter package, emitting 174 ns pulses at 405 ± 5 nm wavelength and a pulse energy of 52 nJ. An aspheric objective with a numerical aperture of 0.60 is used to achieve microscope optical illumination. The laser diode excitation has a compact size of 4.5 × 1.8 × 1.8 cm3 assembled with a cooling block. The lateral resolution was tested to be 0.95 μm on ~7 μm carbon fibers. The subcutaneous microvasculature on a mouse back was label-free imaged ex vivo, which demonstrates the potential of the LD-OR-PAM prototype for in vivo imaging skin chromosphores such as hemoglobin. Our ultimate aim is to provide a practical and affordable OR-PAM system as a routine instrument for standard clinical applications.

Paper Details

Date Published: 15 March 2016
PDF: 4 pages
Proc. SPIE 9708, Photons Plus Ultrasound: Imaging and Sensing 2016, 97084T (15 March 2016); doi: 10.1117/12.2209201
Show Author Affiliations
Lvming Zeng, Jiangxi Science and Technology Normal Univ. (China)
Beckman Laser Institute (United States)
Zhonglie Piao, Beckman Laser Institute (United States)
Shenghai Huang, Beckman Laser Institute (United States)
Wangcun Jia, Beckman Laser Institute (United States)
Zhongping Chen, Beckman Laser Institute (United States)
Univ. of California, Irvine (United States)

Published in SPIE Proceedings Vol. 9708:
Photons Plus Ultrasound: Imaging and Sensing 2016
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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