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Proceedings Paper

Reflectance confocal microscopy of red blood cells: simulation and experiment (Conference Presentation)
Author(s): Adel Zeidan; Daniella Yeheskely-Hayon; Limor Minai; Dvir Yelin

Paper Abstract

The properties of red blood cells are a remarkable indicator of the body's physiological condition; their density could indicate anemia or polycythemia, their absorption spectrum correlates with blood oxygenation, and their morphology is highly sensitive to various pathologic states including iron deficiency, ovalocytosis, and sickle cell disease. Therefore, measuring the morphology of red blood cells is important for clinical diagnosis, providing valuable indications on a patient’s health. In this work, we simulated the appearance of normal red blood cells under a reflectance confocal microscope and discovered unique relations between the cells’ morphological parameters and the resulting characteristic interference patterns. The simulation results showed good agreement with in vitro reflectance confocal images of red blood cells, acquired using spectrally encoded flow cytometry (SEFC) that imaged the cells during linear flow and without artificial staining. By matching the simulated patterns to the SEFC images of the cells, the cells’ three-dimensional shapes were evaluated and their volumes were calculated. Potential applications include measurement of the mean corpuscular volume, cell morphological abnormalities, cell stiffness under mechanical stimuli, and the detection of various hematological diseases.

Paper Details

Date Published: 27 April 2016
PDF: 1 pages
Proc. SPIE 9691, Endoscopic Microscopy XI; and Optical Techniques in Pulmonary Medicine III, 96910N (27 April 2016); doi: 10.1117/12.2209027
Show Author Affiliations
Adel Zeidan, Technion-Israel Institute of Technology (Israel)
Daniella Yeheskely-Hayon, Technion-Israel Institute of Technology (Israel)
Limor Minai, Technion-Israel Institute of Technology (Israel)
Dvir Yelin, Technion-Israel Institute of Technology (Israel)

Published in SPIE Proceedings Vol. 9691:
Endoscopic Microscopy XI; and Optical Techniques in Pulmonary Medicine III
Melissa J. Suter; Guillermo J. Tearney M.D.; Thomas D. Wang; Stephen Lam M.D.; Matthew Brenner, Editor(s)

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