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Proceedings Paper

Multicolor single-molecule imaging by spectral point-spread-function engineering (Conference Presentation)

Paper Abstract

We extend the information content of the microscope’s point-spread-function (PSF) by adding a new degree of freedom: spectral information. We demonstrate controllable encoding of a microscopic emitter’s spectral information (color) and 3D position in the shape of the microscope’s PSF. The design scheme works by exploiting the chromatic dispersion of an optical element placed in the optical path. By using numerical optimization we design a single physical pattern that yields different desired phase delay patterns for different wavelengths. To demonstrate the method’s applicability experimentally, we apply it to super-resolution imaging and to multiple particle tracking.

Paper Details

Date Published: 26 May 2016
PDF: 1 pages
Proc. SPIE 9714, Single Molecule Spectroscopy and Superresolution Imaging IX, 97140L (26 May 2016); doi: 10.1117/12.2208982
Show Author Affiliations
Yoav Shechtman, Stanford Univ. (United States)
Lucien E. Weiss, Stanford Univ. (United States)
Adam S. Backer, Stanford Univ. (United States)
William E. Moerner, Stanford Univ. (United States)

Published in SPIE Proceedings Vol. 9714:
Single Molecule Spectroscopy and Superresolution Imaging IX
Jörg Enderlein; Ingo Gregor; Zygmunt Karol Gryczynski; Rainer Erdmann; Felix Koberling, Editor(s)

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