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Proceedings Paper

Monovalent plasmonic nanoparticles for biological applications
Author(s): Daeha Seo; Hyunjung Lee; Jung-uk Lee; Thomas J. Haas; Young-wook Jun
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Paper Abstract

The multivalent nature of commercial nanoparticle imaging agents and the difficulties associated with producing monovalent nanoparticles challenge their use in biology, where clustering of target biomolecules can perturb dynamics of biomolecular targets. Here, we report production and purification of monovalent gold and silver nanoparticles for their single molecule imaging application. We first synthesized DNA-conjugated 20 nm and 40 nm gold and silver nanoparticles via conventional metal-thiol chemistry, yielding nanoparticles with mixed valency. By employing an anion-exchange high performance liquid chromatography (AE-HPLC) method, we purified monovalent nanoparticles from the mixtures. To allow efficient peak-separation resolution while keeping the excellent colloidal stability of nanoparticles against harsh purification condition (e.g. high NaCl), we optimized surface properties of nanoparticles by modulating surface functional groups. We characterized the monovalent character of the purified nanoparticles by hybridizing two complementary conjugates, forming dimers. Finally, we demonstrate the use of the monovalent plasmonic nanoprobes as single molecule imaging probes by tracking single TrkA receptors diffusing on the cell membrane and compare to monovalent quantum dot probes.

Paper Details

Date Published: 22 April 2016
PDF: 6 pages
Proc. SPIE 9722, Colloidal Nanoparticles for Biomedical Applications XI, 97220I (22 April 2016); doi: 10.1117/12.2208635
Show Author Affiliations
Daeha Seo, Univ. of California, San Francisco (United States)
Lawrence Berkeley National Lab. (United States)
Hyunjung Lee, Univ. of California, San Francisco (United States)
Jung-uk Lee, Univ. of California, San Francisco (United States)
Yonsei Univ. (Korea, Republic of)
Institute for Basic Science (Korea, Republic of)
Thomas J. Haas, Univ. of California, San Francisco (United States)
Young-wook Jun, Univ. of California, San Francisco (United States)

Published in SPIE Proceedings Vol. 9722:
Colloidal Nanoparticles for Biomedical Applications XI
Wolfgang J. Parak; Marek Osinski; Xing-Jie Liang, Editor(s)

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