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Proceedings Paper

Label-free oligonucleotide biosensor based on dual-peak long period fiber grating
Author(s): Xianfeng Chen; Chen Liu; Marcos R. Cardoso; Cleber R. Mendonça; David A. Nagel; Anna V. Hine; Lin Zhang
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Paper Abstract

We report the simplification and development of biofunctionalization methodology based on one-step 1-ethyl-3-(3- dimethylaminopropyl)carbodiimide (EDC)-mediated reaction. The dual-peak long period grating (dLPG) has been demonstrated its inherent ultrahigh sensitivity to refractive index (RI), achieving 50-fold improvement in RI sensitivity over a standard LPG sensor used in low RI range. With the simple and efficient immobilization of unmodified oligonucleotides on sensor surface, dLPG-based biosensor has been used to monitor the hybridization of complementary oligonucleotides showing a detectable oligonucleotide concentration of 4 nM with the advantages of label-free, real-time, and ultrahigh sensitivity.

Paper Details

Date Published: 7 March 2016
PDF: 6 pages
Proc. SPIE 9702, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVI, 97020S (7 March 2016); doi: 10.1117/12.2207869
Show Author Affiliations
Xianfeng Chen, Bangor Univ. (United Kingdom)
Chen Liu, Bangor Univ. (United Kingdom)
Marcos R. Cardoso, Univ. de São Paulo (Brazil)
Cleber R. Mendonça, Univ. de São Paulo (Brazil)
David A. Nagel, Aston Univ. (United Kingdom)
Anna V. Hine, Aston Univ. (United Kingdom)
Lin Zhang, Aston Univ. (United Kingdom)

Published in SPIE Proceedings Vol. 9702:
Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVI
Israel Gannot, Editor(s)

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