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Proceedings Paper

Polarization-dependent single-molecule spectroscopy on photosystem I
Author(s): S. Skandary; A. Konrad; M. Hussels; A. J. Meixner; M. Brecht
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Paper Abstract

Single-molecule spectroscopy (SMS) at low temperature was used to study the spectral properties, heterogeneities and spectral dynamics of the chlorophyll a (Chl a) molecules responsible for the fluorescence emission of photosystem I (PS I). The fluorescence spectra of single PS I complexes are dominated by several red-shifted Chl a molecules categorized into red pools called C708 and C719. By polarization dependent measurements we demonstrate spectrally separate emissions corresponding to C708 and C719 in single PS I monomers and trimers. Moreover, we compared the results of SMS polarization dependent between monomeric and trimeric PS I complexes and give an estimation for the orientation between these red pools. As a consequence, we get new insight into the energy transfer towards and between the red Chl a molecules in PS I complexes.

Paper Details

Date Published: 26 August 2015
PDF: 11 pages
Proc. SPIE 9554, Nanoimaging and Nanospectroscopy III, 95541H (26 August 2015); doi: 10.1117/12.2207523
Show Author Affiliations
S. Skandary, Eberhard Karls Univ. Tübingen (Germany)
A. Konrad, Eberhard Karls Univ. Tübingen (Germany)
M. Hussels, Eberhard Karls Univ. Tübingen (Germany)
A. J. Meixner, Eberhard Karls Univ. Tübingen (Germany)
M. Brecht, Eberhard Karls Univ. Tübingen (Germany)
Zürich Univ. of Applied Sciences (Switzerland)

Published in SPIE Proceedings Vol. 9554:
Nanoimaging and Nanospectroscopy III
Prabhat Verma; Alexander Egner, Editor(s)

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