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Proceedings Paper

Acellular organ scaffolds for tumor tissue engineering
Author(s): Anna Guller; Inna Trusova; Elena Petersen; Anatoly Shekhter; Alexander Kurkov; Yi Qian; Andrei Zvyagin
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Paper Abstract

Rationale: Tissue engineering (TE) is an emerging alternative approach to create models of human malignant tumors for experimental oncology, personalized medicine and drug discovery studies. Being the bottom-up strategy, TE provides an opportunity to control and explore the role of every component of the model system, including cellular populations, supportive scaffolds and signalling molecules.
Objectives: As an initial step to create a new ex vivo TE model of cancer, we optimized protocols to obtain organ-specific acellular matrices and evaluated their potential as TE scaffolds for culture of normal and tumor cells.
Methods and results: Effective decellularization of animals’ kidneys, ureter, lungs, heart, and liver has been achieved by detergent-based processing. The obtained scaffolds demonstrated biocompatibility and growthsupporting potential in combination with normal (Vero, MDCK) and tumor cell lines (C26, B16). Acellular scaffolds and TE constructs have been characterized and compared with morphological methods.
Conclusions: The proposed methodology allows creation of sustainable 3D tumor TE constructs to explore the role of organ-specific cell-matrix interaction in tumorigenesis.

Paper Details

Date Published: 22 December 2015
PDF: 9 pages
Proc. SPIE 9668, Micro+Nano Materials, Devices, and Systems, 96684G (22 December 2015); doi: 10.1117/12.2202473
Show Author Affiliations
Anna Guller, Macquarie Univ. (Australia)
Sechenov First Moscow State Medical Univ. (Russian Federation)
Inna Trusova, Moscow Institute of Physics and Technology (Russian Federation)
Elena Petersen, Moscow Institute of Physics and Technology (Russian Federation)
Anatoly Shekhter, Sechenov First Moscow State Medical Univ. (Russian Federation)
Alexander Kurkov, Sechenov First Moscow State Medical Univ. (Russian Federation)
Yi Qian, Macquarie Univ. (Australia)
Andrei Zvyagin, Macquarie Univ. (Australia)
Nizhny Novgorod State Univ. (Russian Federation)

Published in SPIE Proceedings Vol. 9668:
Micro+Nano Materials, Devices, and Systems
Benjamin J. Eggleton; Stefano Palomba, Editor(s)

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