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Proceedings Paper

Design and validation of a multimodal low-budget Raman microscope for liquid and solid phase applications
Author(s): Hanna Koch; Kristina Noack; Stefan Will
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Paper Abstract

Confocal Raman microscopy is a powerful tool to measure small sample volumes or solids. Since commercial Raman microscopes are expensive and a change of the laser wavelength or the excitation path is hardly possible after the installation, we constructed a multimodal low-budget Raman microscope. Thus, it was possible to significantly increase the flexibility in terms of excitation wavelengths, paths, and planes. Furthermore, the asset costs were reduced by a factor of 1.7. By using commercial as well as home-built objectives to adapt the working distance and the magnification to the system under investigation, the self-constructed Raman microscope offers the possibility to measure big sample volumes, too. The obtained Raman spectra were validated by Raman spectra from a commercial Raman microscope. With a comparable measurement setting it was possible to increase the signal intensities, but with a slightly lower SNR. However, based on the great flexibility of the set-up, e.g., the laser power or the excitation wavelength can be adapted to increase the SNR. Furthermore, measurement times can be decreased. With this low-budget self-constructed Raman microscope high quality Raman microscopy and micro spectroscopy can be performed with a high flexibility to fast adapt the set-up to the sample under investigation which is not offered by commercial microscopes.

Paper Details

Date Published: 22 June 2015
PDF: 8 pages
Proc. SPIE 9529, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials II, 952917 (22 June 2015); doi: 10.1117/12.2184571
Show Author Affiliations
Hanna Koch, Lehrstuhl für Technische Thermodynamik (Germany)
Kristina Noack, Lehrstuhl für Technische Thermodynamik (Germany)
Stefan Will, Lehrstuhl für Technische Thermodynamik (Germany)

Published in SPIE Proceedings Vol. 9529:
Optical Methods for Inspection, Characterization, and Imaging of Biomaterials II
Pietro Ferraro; Simonetta Grilli; Monika Ritsch-Marte; David Stifter, Editor(s)

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