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Proceedings Paper

Accurate and in situ monitoring of bacterial concentration using a real time all-fibre spectroscopic device
Author(s): W. Tao; C. McGoverin; S. Lydiard; Y. Song; M. Cheng; S. Swift; N. Singhal; F. Vanholsbeeck
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Paper Abstract

Accurate in situ monitoring of bacterial transport is important for increased understanding and improvement of bioremediation processes where microorganisms convert toxic compounds to more benign compounds. Bioremediation methods have become the preferred mechanism for the rehabilitation of hard to reach contaminated environments. In this study, we have used fluorescence spectroscopy to monitor the movement of fluorescently labelled bacteria (Rhodococcus erythropolis and Pseudomonas putida) within a bench-top column filled with a porous medium. In situ fluorescence measurements made using a fibre optic based instrument (‘optrode’) were compared to ex situ measurements made using a plate reader. In situ monitoring using this fibre optic based instrument is a promising alternative to ex situ measurements as the initial flow of bacteria is reliably observed. However, a greater understanding of the effect of the porous medium on fluorescence measurements is required to develop an accurate calibration for bacterial concentration based in situ measurements.

Paper Details

Date Published: 1 July 2015
PDF: 5 pages
Proc. SPIE 9655, Fifth Asia-Pacific Optical Sensors Conference, 96551J (1 July 2015); doi: 10.1117/12.2184388
Show Author Affiliations
W. Tao, The Univ. of Auckland (New Zealand)
C. McGoverin, The Univ. of Auckland (New Zealand)
S. Lydiard, The Univ. of Auckland (New Zealand)
Y. Song, The Univ. of Auckland (New Zealand)
M. Cheng, The Univ. of Auckland (New Zealand)
S. Swift, The Univ. of Auckland (New Zealand)
N. Singhal, The Univ. of Auckland (New Zealand)
F. Vanholsbeeck, The Univ. of Auckland (New Zealand)

Published in SPIE Proceedings Vol. 9655:
Fifth Asia-Pacific Optical Sensors Conference
Byoungho Lee; Sang-Bae Lee; Yunjiang Rao, Editor(s)

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