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Proceedings Paper

Characterization of atherosclerotic arterial tissue using combined SHG and FLIM microscopy
Author(s): Riccardo Cicchi; Enrico Baria; Christian Matthäus; Marta Lange; Annika Lattermann; Bernhard R. Brehm; Jürgen Popp; Francesco S. Pavone
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Paper Abstract

Atherosclerosis is among the most widespread cardiovascular diseases and one of the leading cause of death in the Western World. Characterization of arterial tissue in atherosclerotic condition is extremely interesting from the diagnostic point of view, especially for what is concerning collagen content and organization because collagen plays a crucial role in plaque vulnerability. Routinely used diagnostic methods, such as histopathological examination, are limited to morphological analysis of the examined tissues, whereas an exhaustive characterization requires immunehistochemical examination and a morpho-functional approach. Non-linear microscopy techniques offer the potential for providing morpho-functional information on the examined tissues in a label-free way. In this study, we employed combined SHG and FLIM microscopy for characterizing collagen organization in both normal arterial wall and within atherosclerotic plaques. Image pattern analysis of SHG images allowed characterizing collagen organization in different tissue regions. In addition, the analysis of collagen fluorescence decay contributed to the characterization of the samples based on collagen fluorescence lifetime. Different values of collagen fiber mean size, collagen distribution, and collagen anisotropy and collagen fluorescence lifetime were found in normal arterial wall and within plaque depositions, prospectively allowing for automated classification of atherosclerotic lesions and plaque vulnerability. The presented method represents a promising diagnostic tool for evaluating atherosclerotic tissue and has the potential to find a stable place in clinical setting as well as to be applied in vivo in the near future.

Paper Details

Date Published: 14 July 2015
PDF: 4 pages
Proc. SPIE 9536, Advanced Microscopy Techniques IV; and Neurophotonics II, 95360N (14 July 2015); doi: 10.1117/12.2183623
Show Author Affiliations
Riccardo Cicchi, National Institute of Optics, INO-CNR (Italy)
European Lab. for Non-Linear Spectroscopy (Italy)
Enrico Baria, European Lab. for Non-Linear Spectroscopy (Italy)
Christian Matthäus, Institute of Photonic Technology (Germany)
Marta Lange, Institute of Biomedical Engineering and Nanotechnology (Latvia)
Annika Lattermann, Jena Univ. Hospital, Friedrich-Schiller-Univ. (Germany)
Bernhard R. Brehm, Herz-Neuro-Zentrum Bodensee (Switzerland)
Jürgen Popp, Institute of Photonic Technology (Germany)
Friedrich Schiller Univ. Jena (Germany)
Francesco S. Pavone, National Institute of Optics, INO-CNR (Italy)
European Lab. for Non-Linear Spectroscopy (Italy)
Univ. of Florence (Italy)

Published in SPIE Proceedings Vol. 9536:
Advanced Microscopy Techniques IV; and Neurophotonics II
Emmanuel Beaurepaire; Francesco Pavone; Elizabeth M. Hillman; Peter T. C. So, Editor(s)

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