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Proceedings Paper

3D imaging of apoptosis by FRET, light sheet fluorescence and scattering microsopy
Author(s): Herbert Schneckenburger; Petra Weber; Sarah Schickinger; Verena Richter; Thomas Bruns; Michael Wagner
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Paper Abstract

Non-radiative cell membrane associated energy transfer (FRET) from a cyan (ECFP) to a yellow (EYFP) fluorescent protein is used for detection of apoptosis in multi-cellular tumor spheroids. Low light exposure in light sheet fluorescence microscopy is combined with the detection of spectral changes and prolonged fluorescence lifetimes of the donor ECFP upon apoptosis. In view of future label-free detection preliminary experiments of light scattering microscopy with high angular resolution are reported which may give additional information on morphological changes.

Paper Details

Date Published: 15 July 2015
PDF: 5 pages
Proc. SPIE 9537, Clinical and Biomedical Spectroscopy and Imaging IV, 953716 (15 July 2015); doi: 10.1117/12.2183607
Show Author Affiliations
Herbert Schneckenburger, Aalen Univ. (Germany)
Petra Weber, Aalen Univ. (Germany)
Sarah Schickinger, Aalen Univ. (Germany)
Verena Richter, Aalen Univ. (Germany)
Thomas Bruns, Aalen Univ. (Germany)
Michael Wagner, Aalen Univ. (Germany)

Published in SPIE Proceedings Vol. 9537:
Clinical and Biomedical Spectroscopy and Imaging IV
J. Quincy Brown; Volker Deckert, Editor(s)

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