The transverse and longitudinal plasmon resonance in gold nanorods can be exploited to localize the photothermal therapy and influence the fluorescence to monitor the treatment outcome at the same time. While the longitudinal plasmon peak contributes to the photothermal effect, the transverse peak can enhance fluorescence. After cells take in PEGylated nanorods through endocytosis, autofluorescence from endogenous fluorophores such as nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) in the mitochondria is enhanced two times, which is a good indicator of the respiratory status of the cell. When cells are illuminated continuously with near infrared laser, the temperature reaches the hyperthermic region within the first four minutes, which demonstrates the efficiency of gold nanorods in photothermal therapy. The cell viability test and autofluorescence intensity show good correlation indicating the progress of cell death over time.

Date Published: 15 July 2015
PDF: 6 pages
Proc. SPIE 9537, Clinical and Biomedical Spectroscopy and Imaging IV, 95371B (15 July 2015); doi: 10.1117/12.2183581

Published in SPIE Proceedings Vol. 9537:
Clinical and Biomedical Spectroscopy and Imaging IV
J. Quincy Brown; Volker Deckert, Editor(s)