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Proceedings Paper • Open Access

Nuclear dynamics in metastatic cells studied by quantitative phase imaging

Paper Abstract

We used a new quantitative high spatiotemporal resolution phase imaging tool to explore the nuclear structure and dynamics of individual cells. We used a novel analysis tool to quantify the diffusion outside and inside the nucleus of live cells. We also obtained information about the nuclear spatio temporal mass density in metastatic cells. The results indicate that in the cytoplasm, the intracellular transport is mainly active (direct, deterministic), while inside the nucleus it is both active and passive (diffusive, random). We calculated the standard deviation of velocities in active transport and the diffusion coefficient for passive transport.

Paper Details

Date Published: 11 March 2015
PDF: 5 pages
Proc. SPIE 9336, Quantitative Phase Imaging, 93361Q (11 March 2015); doi: 10.1117/12.2183296
Show Author Affiliations
Silvia Ceballos, Univ. of Illinois at Urbana-Champaign (United States)
National Univ. of Colombia (Colombia)
Mikhail Kandel, Univ. of Illinois at Urbana-Champaign (United States)
Shamira Sridharan, Univ. of Illinois at Urbana-Champaign (United States)
Freddy Monroy, National Univ. of Columbia (Colombia)
Gabriel Popescu, Univ. of Illinois at Urbana-Champaign (United States)

Published in SPIE Proceedings Vol. 9336:
Quantitative Phase Imaging
Gabriel Popescu; YongKeun Park, Editor(s)

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