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Proceedings Paper

Hardware-friendly bi-exponential fluorescence lifetime imaging algorithms and phasor approaches
Author(s): David Li; Yu Chen
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Paper Abstract

A newly developed hardware-friendly non-iterative fluorescence lifetime imaging (FLIM) analysis method was verified in an FPGA chip. Its performances were also demonstrated on two-photon FLIM images of gold nanorods (GNRs)-Cy5 labelled Hela cells. The results obtained by the proposed method can be presented in a polor plot to be compared to the widely used phasor (Phasor) approach. Combining our method with Phasor will be very useful in FLIM analysis.

Paper Details

Date Published: 14 July 2015
PDF: 5 pages
Proc. SPIE 9536, Advanced Microscopy Techniques IV; and Neurophotonics II, 95360M (14 July 2015); doi: 10.1117/12.2183082
Show Author Affiliations
David Li, Univ. of Strathclyde (United Kingdom)
Yu Chen, Univ. of Strathclyde (United Kingdom)

Published in SPIE Proceedings Vol. 9536:
Advanced Microscopy Techniques IV; and Neurophotonics II
Emmanuel Beaurepaire; Francesco Pavone; Elizabeth M. Hillman; Peter T. C. So, Editor(s)

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