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Proceedings Paper

Implications of the focal beam profile in serial femtosecond crystallography
Author(s): Lorenzo Galli; Peter Metcalf; Henry N. Chapman
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Paper Abstract

The photon density profile of an X-ray free-electron laser (XFEL) beam at the focal position is a critical parameter for serial femtosecond crystallography (SFX), but is difficult to measure because of the destructive power of the beam. A novel high intensity radiation induced phasing method (HIRIP) has been proposed as a general experimental approach for protein structure determination, but has proved to be sensitive to variations of the X-ray intensity, with uniform incident fluence desired for best performance. Here we show that experimental SFX data collected at the nano-focus chamber of the Coherent X-ray Imaging end-station at the Linac Coherent Light Source using crystals with a limited size distribution suggests an average profile of the X-ray beam that has a large variation of intensity. We propose a new method to improve the quality of high fluence data for HI-RIP, by identifying and removing diffraction patterns from crystals exposed to the low intensity region of the beam. The method requires crystals of average size comparable to the width of the focal spot.

Paper Details

Date Published: 12 May 2015
PDF: 7 pages
Proc. SPIE 9511, Damage to VUV, EUV, and X-ray Optics V, 95110H (12 May 2015); doi: 10.1117/12.2182654
Show Author Affiliations
Lorenzo Galli, Ctr. for Free-Electron Laser Science (Germany)
Univ. of Hamburg (Germany)
Peter Metcalf, Univ. of Auckland (New Zealand)
Henry N. Chapman, Ctr. for Free-Electron Laser Science (Germany)
Univ. of Hamburg (Germany)
The Hamburg Ctr. for Ultrafast Imaging (Germany)

Published in SPIE Proceedings Vol. 9511:
Damage to VUV, EUV, and X-ray Optics V
Libor Juha; Saša Bajt; Richard London, Editor(s)

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