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Proceedings Paper

A SERS-based microfluidic immunoassay using an in-situ synthesized gold substrate
Author(s): Kequan Fan; Zhuyuan Wang; Lei Wu; Shenfei Zong; Yiping Cui
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Paper Abstract

A sensitive SERS (surface-enhanced Raman scattering)-based immunoassay in microfluidic system has been developed with in-situ synthesis of gold substrate and immune reporter named as 4MBA (4-Mercaptobenzoic acid)-labeled immuno-Ag aggregates. The gold substrate was fabricated simply by introducing the hydrogen tetrachloroaurate (III) trihydrate (HAuCl4) solution to microchannels using a microfluidic pump. It was found that the obtained deposited gold nanoparticles were uniform in size and shape. Then the sandwich immunoassays were performed using the gold substrates based on SERS signals. In the immunoassay, the gold nanoparticles decorated surface was modified with certain antibodies to recognize the specific kind of antigen, which was flowed through the microfluidic channel afterwards. Then 4MBA-labeled immuno-Ag aggregates were employed as the SERS probes to quantitatively detect the antigen. The experimental results showed a good specificity and limit of detection (LOD) about 1 ng/mL.

Paper Details

Date Published: 4 May 2015
PDF: 7 pages
Proc. SPIE 9543, Third International Symposium on Laser Interaction with Matter, 954319 (4 May 2015); doi: 10.1117/12.2182132
Show Author Affiliations
Kequan Fan, Southeast Univ. (China)
Zhuyuan Wang, Southeast Univ. (China)
Lei Wu, Southeast Univ. (China)
Shenfei Zong, Southeast Univ. (China)
Yiping Cui, Southeast Univ. (China)

Published in SPIE Proceedings Vol. 9543:
Third International Symposium on Laser Interaction with Matter
Yury M. Andreev; Zunqi Lin III; Xiaowu Ni; Xisheng Ye, Editor(s)

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