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Proceedings Paper

A novel measurement method of microorganism growth by tunable diode laser-absorption spectroscopy
Author(s): Jindong Xiang; Jie Shao; Chaofu Ying; Liming Wang; Jie Guo
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Paper Abstract

The objective of this work was to attain essential parameters by using a Gompertz model that employed a new approach of wavelength modulation spectroscopy (WMS) to describe the microorganism growth. The measurement method of WMS introduces noninvasive technique instead of complicated invasive microorganism operation analysis and quickly obtains the accurate real-time measurement results. By using the WMS measurement, the specific growth curve of microorganism growth clearly displayed every three minute, which has characteristics of high sensitivity, high spectral resolution, fast time response and overcomes the randomness and error operation of traditional analysis methods. The measurement value of BF and AF in the range of 1.008 to 1.043 and the lower MSE showed that Gompertz model can fit the data well and be capable of describing bacteria growth rate and lag time. The results of experiment data suggested that the specific growth rate of microorganism depends on the temperature. With the increase of temperature ranging from 25 °C to 42 °C , the lag time of bacteria growth has been shortened. And the suitable temperature of bacteria growth is about 37 °C . Judging from the growth rate of microorganisms, we can identify the microbial species, not only to improve the precision and efficiency, but also to provides a rapidly sensitive way for microbial detection. The lag time of microorganism growth also provides a great application prospect for shelf life of the food safety.

Paper Details

Date Published: 4 May 2015
PDF: 12 pages
Proc. SPIE 9543, Third International Symposium on Laser Interaction with Matter, 954315 (4 May 2015); doi: 10.1117/12.2181843
Show Author Affiliations
Jindong Xiang, Zhejiang Normal Univ. (China)
Jie Shao, Zhejiang Normal Univ. (China)
Chaofu Ying, Zhejiang Normal Univ. (China)
Liming Wang, Zhejiang Normal Univ. (China)
Jie Guo, Zhejiang Normal Univ. (China)

Published in SPIE Proceedings Vol. 9543:
Third International Symposium on Laser Interaction with Matter
Yury M. Andreev; Zunqi Lin III; Xiaowu Ni; Xisheng Ye, Editor(s)

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