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Proceedings Paper

Methylene blue photodynamic therapy in rats' wound healing: 21 days follow-up
Author(s): Vanda Sanderana Macêdo Carneiro; Maria Helena Chaves de Vasconcelos Catao; Rebeca Ferraz Menezes; Natália Costa Araújo; Marleny Elizabeth Martinez Gerbi
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Paper Abstract

The experimental evaluated the photodynamic therapy (PDT) in wound healing. It used 60 male rats, making two circular wounds at each animal. They were treated at 48hs intervals, with methylene blue (MB), low level laser treatment (LLLT) or both, thus resulting in PDT. The wounds were observed 01, 03, 07, 14 and 21 days after and then processed and subjected to HE staining to analyze granulation tissue, necrosis, epithelialization and collagen. After day 1, wounds treated with MB showed necrosis less intense than other groups, and the PDT group showed more intense granulation tissue. At day 3, reepithelialization was absent for half of injuries in the PDT group, and this group was also with lower collagen. However, at day 7, this same group presented reepithelialization more advanced than control group, which did not happen with those treated with MB or LLLT (p = 0.015). The results allow us to conclude that PDT difficulted reepithelization at 7th day and interfered in standard healing. However, when used separately, MB and LLLT interfered significantly compared to the control group, which did not happened to the PDT group. There was no significant difference between the treatment groups in other analysed times.

Paper Details

Date Published: 19 June 2015
PDF: 7 pages
Proc. SPIE 9531, Biophotonics South America, 95311S (19 June 2015); doi: 10.1117/12.2181124
Show Author Affiliations
Vanda Sanderana Macêdo Carneiro, The Univ. of Pernambuco (Brazil)
Maria Helena Chaves de Vasconcelos Catao, Univ. Federal da Paraíba (Brazil)
Rebeca Ferraz Menezes, The Univ. of Pernambuco (Brazil)
Natália Costa Araújo, The Univ. of Pernambuco (Brazil)
Marleny Elizabeth Martinez Gerbi, The Univ. of Pernambuco (Brazil)

Published in SPIE Proceedings Vol. 9531:
Biophotonics South America
Cristina Kurachi D.D.S.; Katarina Svanberg M.D.; Bruce J. Tromberg; Vanderlei Salvador Bagnato, Editor(s)

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