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Proceedings Paper

Confocal Raman study of aging process in diabetes mellitus human voluntaries
Author(s): Liliane Pereira; Claudio Alberto Téllez Soto; Laurita dos Santos; Syed Mohammed Ali; Priscila Pereira Fávero; Airton A. Martin
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Paper Abstract

Accumulation of AGEs [Advanced Glycation End – products] occurs slowly during the human aging process. However, its formation is accelerated in the presence of diabetes mellitus. In this paper, we perform a noninvasive analysis of glycation effect on human skin by in vivo confocal Raman spectroscopy. This technique uses a laser of 785 nm as excitation source and, by the inelastic scattering of light, it is possible to obtain information about the biochemical composition of the skin. Our aim in this work was to characterize the aging process resulting from the glycation process in a group of 10 Health Elderly Women (HEW) and 10 Diabetic Elderly Women (DEW). The Raman data were collected from the dermis at a depth of 70-130 microns. Through the theory of functional density (DFT) the bands positions of hydroxyproline, proline and AGEs (pentosidine and glucosepane) were calculated by using Gaussian 0.9 software. A molecular interpretation of changes in type I collagen was performed by the changes in the vibrational modes of the proline (P) and hydroxyproline (HP). The data analysis shows that the aging effects caused by glycation of proteins degrades type I collagen differently and leads to accelerated aging process.

Paper Details

Date Published: 19 June 2015
PDF: 9 pages
Proc. SPIE 9531, Biophotonics South America, 95312M (19 June 2015); doi: 10.1117/12.2181040
Show Author Affiliations
Liliane Pereira, Univ. do Vale do Paraíba (Brazil)
Claudio Alberto Téllez Soto, Univ. do Vale do Paraíba (Brazil)
Laurita dos Santos, Univ. do Vale do Paraíba (Brazil)
Syed Mohammed Ali, Univ. do Vale do Paraíba (Brazil)
Priscila Pereira Fávero, Univ. do Vale do Paraíba (Brazil)
Airton A. Martin, Univ. do Vale do Paraíba (Brazil)

Published in SPIE Proceedings Vol. 9531:
Biophotonics South America
Cristina Kurachi D.D.S.; Katarina Svanberg M.D.; Bruce J. Tromberg; Vanderlei Salvador Bagnato, Editor(s)

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