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Proceedings Paper

Analysis of the in vivo confocal Raman spectral variability in human skin
Author(s): Borys Mogilevych; Laurita dos Santos; Joao L. Rangel; Karen J. S. Grancianinov; Mariane P. Sousa; Airton A. Martin
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Paper Abstract

Biochemical composition of the skin changes in each layer and, therefore, the skin spectral profile vary with the depth. In this work, in vivo Confocal Raman spectroscopy studies were performed at different skin regions and depth profile (from the surface down to 10 μm) of the stratum corneum, to verify the variability and reproducibility of the intra- and interindividual Raman data. The Raman spectra were collected from seven healthy female study participants using a confocal Raman system from Rivers Diagnostic, with 785 nm excitation line and a CCD detector. Measurements were performed in the volar forearm region, at three different points at different depth, with the step of 2 μm. For each depth point, three spectra were acquired. Data analysis included the descriptive statistics (mean, standard deviation and residual) and Pearson's correlation coefficient calculation. Our results show that inter-individual variability is higher than intraindividual variability, and variability inside the SC is higher than on the skin surface. In all these cases we obtained r values, higher than 0.94, which correspond to high correlation between Raman spectra. It reinforces the possibility of the data reproducibility and direct comparison of in vivo results obtained with different study participants of the same age group and phototype.

Paper Details

Date Published: 19 June 2015
PDF: 8 pages
Proc. SPIE 9531, Biophotonics South America, 95312F (19 June 2015); doi: 10.1117/12.2181030
Show Author Affiliations
Borys Mogilevych, Univ. do Vale do Paraiba (Brazil)
Laurita dos Santos, Univ. do Vale do Paraíba (Brazil)
Joao L. Rangel, Univ. do Vale do Paraíba (Brazil)
Karen J. S. Grancianinov, Univ. do Vale do Paraíba (Brazil)
Mariane P. Sousa, Univ. do Vale do Paraíba (Brazil)
Airton A. Martin, Univ. do Vale do Paraíba (Brazil)

Published in SPIE Proceedings Vol. 9531:
Biophotonics South America
Cristina Kurachi D.D.S.; Katarina Svanberg M.D.; Bruce J. Tromberg; Vanderlei Salvador Bagnato, Editor(s)

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