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Proceedings Paper

Portable widefield imaging device for ICG-detection of the sentinel lymph node
Author(s): Angelo Biasi Govone; Pablo Aurelio Gómez-García; André Lopes Carvalho; Renato de Castro Capuzzo; Daniel Varela Magalhães; Cristina Kurachi
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Paper Abstract

Metastasis is one of the major cancer complications, since the malignant cells detach from the primary tumor and reaches other organs or tissues. The sentinel lymph node (SLN) is the first lymphatic structure to be affected by the malignant cells, but its location is still a great challenge for the medical team. This occurs due to the fact that the lymph nodes are located between the muscle fibers, making it visualization difficult. Seeking to aid the surgeon in the detection of the SLN, the present study aims to develop a widefield fluorescence imaging device using the indocyanine green as fluorescence marker. The system is basically composed of a 780nm illumination unit, optical components for 810nm fluorescence detection, two CCD cameras, a laptop, and dedicated software. The illumination unit has 16 diode lasers. A dichroic mirror and bandpass filters select and deliver the excitation light to the interrogated tissue, and select and deliver the fluorescence light to the camera. One camera is responsible for the acquisition of visible light and the other one for the acquisition of the ICG fluorescence. The software developed at the LabVIEW® platform generates a real time merged image where it is possible to observe the fluorescence spots, related to the lymph nodes, superimposed at the image under white light. The system was tested in a mice model, and a first patient with tongue cancer was imaged. Both results showed the potential use of the presented fluorescence imaging system assembled for sentinel lymph node detection.

Paper Details

Date Published: 19 June 2015
PDF: 7 pages
Proc. SPIE 9531, Biophotonics South America, 95312D (19 June 2015); doi: 10.1117/12.2181025
Show Author Affiliations
Angelo Biasi Govone, Univ. de São Paulo (Brazil)
Pablo Aurelio Gómez-García, Univ. de São Paulo (Brazil)
André Lopes Carvalho, Barretos Cancer Hospital (Brazil)
Renato de Castro Capuzzo, Barretos Cancer Hospital (Brazil)
Daniel Varela Magalhães, Univ. de São Paulo (Brazil)
Cristina Kurachi, Univ. de São Paulo (Brazil)

Published in SPIE Proceedings Vol. 9531:
Biophotonics South America
Cristina Kurachi D.D.S.; Katarina Svanberg M.D.; Bruce J. Tromberg; Vanderlei Salvador Bagnato, Editor(s)

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