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Proceedings Paper

Preparation of HIV monoclonal antibody-conjugated pulchellin in order to study its intracellular trafficking pathway in HIV-infected cells by confocal microscopy
Author(s): M. Sadraeian; F. M. Tsutae; H. H. T. Moreira; A. P. U. Araujo; F. E. G. Guimarães; S. H. Pincus
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Paper Abstract

Pulchellin is a type 2 of ribosome-inactivating proteins isolated from some seeds significantly growing in Brazil. It is a potent agent to inhibit the protein synthesis in cancer cells and also HIV-infected cells. Pulchellin can be conjugated to HIV monoclonal antibodies to specifically target the HIV-infected cells. To analyze the protein synthesis inhibition by Pulchellin, the intracellular localization of the immunoconjugate should be compared to Pulchellin. In this case, the intracellular trafficking of this protein in cells can be determined by confocal microscopy. In our study, we utilized Pulchellin to construct HIV monoclonal antibody-conjugated Pulchellin A chain in order to target HIV-infected lymphocyte cells. Afterward the conjugation was labeled with the superior Alexa Fluor 488 dye. As a subsequent step, we are interested in studying the intracellular trafficking pathway of this novel conjugation in HIV-infected cells by confocal microscopy. Moreover, possible quantitative methods for fluorescent labeling of the immunoconjugate during confocal microscopy will be investigated.

Paper Details

Date Published: 19 June 2015
PDF: 7 pages
Proc. SPIE 9531, Biophotonics South America, 953117 (19 June 2015); doi: 10.1117/12.2181011
Show Author Affiliations
M. Sadraeian, Univ. of São Paulo (Brazil)
F. M. Tsutae, Univ. of São Paulo (Brazil)
H. H. T. Moreira, Univ. of São Paulo (Brazil)
A. P. U. Araujo, Univ. of São Paulo (Brazil)
F. E. G. Guimarães, Univ. of São Paulo (Brazil)
S. H. Pincus, Louisiana State Univ. (United States)

Published in SPIE Proceedings Vol. 9531:
Biophotonics South America
Cristina Kurachi D.D.S.; Katarina Svanberg M.D.; Bruce J. Tromberg; Vanderlei Salvador Bagnato, Editor(s)

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