Diffuse reflectance spectroscopy (DRS) with a fiber-optic contact probe is a cost-effective, rapid, and non-invasive optical method used to extract diagnosis information of tissue. By combining commercially available VIS- and NIR-spectrometers with various fiber-optic contact-probes, we have access to the full wavelength range from around 400 to 1600 nm. Using this flexible and portable spectroscopy system, we have acquired ex-vivo DRS-spectra from murine, porcine, and human liver tissue. For extracting the tissue optical properties from the measured spectra, we have employed and compared predictions from two models for light propagation in tissue, diffusion theory model (DT) and Monte Carlo simulations (MC).

The focus in this work is on the capacity of this DRS-technique in discriminating metastatic tumor tissue from normal liver tissue as well as in assessing and characterizing damage to non-malignant liver tissue induced by preoperative chemotherapy for colorectal liver metastases.

Date Published: 19 June 2015
PDF: 10 pages
Proc. SPIE 9531, Biophotonics South America, 95314E (19 June 2015); doi: 10.1117/12.2181008

Published in SPIE Proceedings Vol. 9531:
Biophotonics South America
Cristina Kurachi D.D.S.; Katarina Svanberg M.D.; Bruce J. Tromberg; Vanderlei Salvador Bagnato, Editor(s)