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Proceedings Paper

Image correlation based method for the analysis of collagen fibers patterns
Author(s): Ramon G. T. Rosa; Sebastião Pratavieira; Cristina Kurachi
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Paper Abstract

The collagen fibers are one of the most important structural proteins in skin, being responsible for its strength and flexibility. It is known that their properties, like fibers density, ordination and mean diameter can be affected by several skin conditions, what makes these properties a good parameter to be used on the diagnosis and evaluation of skin aging, cancer, healing, among other conditions. There is, however, a need for methods capable of analyzing quantitatively the organization patterns of these fibers. To address this need, we developed a method based on the autocorrelation function of the images that allows the construction of vector field plots of the fibers directions and does not require any kind of curve fitting or optimization. The analyzed images were obtained through Second Harmonic Generation Imaging Microscopy. This paper presents a concise review on the autocorrelation function and some of its applications to image processing, details the developed method and the results obtained through the analysis of hystopathological slides of landrace porcine skin. The method has high accuracy on the determination of the fibers direction and presents high performance. We look forward to perform further studies keeping track of different skin conditions over time.

Paper Details

Date Published: 19 June 2015
PDF: 9 pages
Proc. SPIE 9531, Biophotonics South America, 95310B (19 June 2015); doi: 10.1117/12.2180920
Show Author Affiliations
Ramon G. T. Rosa, Univ. of São Paulo (Brazil)
Sebastião Pratavieira, Univ. of São Paulo (Brazil)
Cristina Kurachi, Univ. of São Paulo (Brazil)

Published in SPIE Proceedings Vol. 9531:
Biophotonics South America
Cristina Kurachi D.D.S.; Katarina Svanberg M.D.; Bruce J. Tromberg; Vanderlei Salvador Bagnato, Editor(s)

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