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Proceedings Paper

Hyperspectral light field imaging
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Paper Abstract

A light field camera acquires the intensity and direction of rays from a scene providing a 4D representation L(x,y,u,v) called the light field. The acquired light field allows to virtually change view point and selectively re-focus regions algorithmically, an important feature for many applications in imaging and microscopy. The combination with hyperspectral imaging provides the additional advantage that small objects (beads, cells, nuclei) can be categorised using their spectroscopic signatures. Using an inverse fluorescence microscope, a LCTF tuneable filter and a light field setup as a test-bed, fluorescence-marked beads have been imaged and reconstructed into a 4D hyper-spectral image cube LHSI(x,y,z,λ). The results demonstrate the advantages of the approach for fluorescence microscopy providing extended depth of focus (DoF) and the fidelity of hyper-spectral imaging.

Paper Details

Date Published: 5 May 2015
PDF: 8 pages
Proc. SPIE 9506, Optical Sensors 2015, 950605 (5 May 2015); doi: 10.1117/12.2179463
Show Author Affiliations
Raimund Leitner, CTR AG (Austria)
Andreas Kenda, CTR AG (Austria)
Andreas Tortschanoff, CTR AG (Austria)

Published in SPIE Proceedings Vol. 9506:
Optical Sensors 2015
Francesco Baldini; Jiri Homola; Robert A. Lieberman, Editor(s)

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