Digital holographic microscopy has numerous applications in biology for visualizing living cells and 3D tissues. This technique allows for the direct visualization of biological structures avoiding invasive and phototoxic procedures such as fixation and dying processing. In this study we have characterized the morphometry changes of neurons subject to osmolarity changes. For this purpose, we have measured the variations of the amplitude and the oscillation frequency of the plasmatic membrane, as well as the volume changes of the cells before the osmotic shock. There was a relation between the neural culture ageing and its behavioral changes. "Long-term" cultures that had not previously been studied were used to analyze the behavioral changes in aged cells.

Date Published: 8 May 2015
PDF: 7 pages
Proc. SPIE 9508, Holography: Advances and Modern Trends IV, 950803 (8 May 2015); doi: 10.1117/12.2179116

Published in SPIE Proceedings Vol. 9508:
Holography: Advances and Modern Trends IV
Miroslav Hrabovský; John T. Sheridan; Antonio Fimia, Editor(s)