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Proceedings Paper

Lab-on-a-chip enabled HLA diagnostic: combined sample preparation and real time PCR for HLA-B57 diagnosis
Author(s): Claudia Gärtner; Holger Becker; Nadine Hlawatsch; Richard Klemm; Christian Moche; Sebastian Schattschneider; Rainer Frank; Andreas Willems
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Paper Abstract

The diverse human HLA (human leukocyte antigen) system is responsible for antigen presentation and recognition. It is essential for the immune system to maintain a stable defense line, but also is also involved in autoimmunity as well as metabolic disease. HLA-haplotype (HLA-B27), for instance, is associated with inflammatory diseases such as Bechterew's disease. The administration of the HIV drug Abacavir in combination with another HLA-haplotype (HLAB57) is associated with severe hypersensitivity reactions. Accordingly, the HLA status has to be monitored for diagnosis or prior to start of therapy. Along this line, a miniaturized microfluidic platform has been developed allowing performing the complete analytical process from “sample-in” to “answer-out” in a point-of-care environment. The main steps of the analytical cascade inside the integrated system are blood cell lysis and DNA isolation, DNA purification, real-time PCR and quantitative monitoring of the rise of a fluorescent signal appearing during the PCR based sequence amplification. All bio-analytical steps were intended to be performed inside one chip and will be actuated, controlled and monitored by a matching device. This report will show that all required processes are established and tested and all device components work well and interact with the functional modules on the chips in a harmonized fashion.

Paper Details

Date Published: 13 May 2015
PDF: 5 pages
Proc. SPIE 9490, Advances in Global Health through Sensing Technologies 2015, 94900F (13 May 2015); doi: 10.1117/12.2178897
Show Author Affiliations
Claudia Gärtner, microfluidic ChipShop GmbH (Germany)
Holger Becker, microfluidic ChipShop GmbH (Germany)
Nadine Hlawatsch, microfluidic ChipShop GmbH (Germany)
Richard Klemm, microfluidic ChipShop GmbH (Germany)
Christian Moche, microfluidic ChipShop GmbH (Germany)
Sebastian Schattschneider, microfluidic ChipShop GmbH (Germany)
Rainer Frank, inno-train Diagnostik GmbH (Germany)
Andreas Willems, inno-train Diagnostik GmbH (Germany)

Published in SPIE Proceedings Vol. 9490:
Advances in Global Health through Sensing Technologies 2015
Šárka O. Southern; Mark A. Mentzer; Virginia E. Wotring, Editor(s)

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