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Proceedings Paper

Simultaneous detection of tissue autofluorescence decay distribution and time-gated photo-bleaching rates
Author(s): Alexey Lihachev; Inesa Ferulova; Janis Spigulis; Mindaugas Tamosiunas
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Paper Abstract

Experimental methodology for parallel measurements of in-vivo skin autofluorescence (AF) lifetimes and photobleaching dynamic has been developed and tested. The AF lifetime decay distributions were periodically collected from fixed tissue area with subsequent detection of the fluorescence intensity decrease dynamic at different time gates after the pulse excitation. Temporal distributions of human in-vivo skin AF lifetimes and bleaching kinetics were collected and analyzed by means of commercial time-correlated single photon counting system.

Paper Details

Date Published: 6 May 2015
PDF: 6 pages
Proc. SPIE 9504, Photon Counting Applications 2015, 95040P (6 May 2015); doi: 10.1117/12.2178827
Show Author Affiliations
Alexey Lihachev, Univ. of Latvia (Latvia)
Inesa Ferulova, Univ. of Latvia (Latvia)
Janis Spigulis, Univ. of Latvia (Latvia)
Mindaugas Tamosiunas, Vytautas Magnus Univ. (Lithuania)

Published in SPIE Proceedings Vol. 9504:
Photon Counting Applications 2015
Ivan Prochazka; Roman Sobolewski; Ralph B. James, Editor(s)

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