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Proceedings Paper

The development of multi incident angles and multi points measurement phase image interrogation surface plasmon resonance system
Author(s): Jyun Liao; Shu-Sheng Lee; Shih-Yuan Lin
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Paper Abstract

Surface plasmon resonance (SPR) is one of the recent applied technologies in bio-medical detection, and it is gradually accepted by the researchers. However, it is still not adopted widely and needs more efforts to improve. In our research work, a previous developed phase interrogation SPR detection system is modified and the concept of multi-incident angles of detecting light is used for obtaining more data. Besides, using the focusing characteristic of a cylindrical elliptic reflective mirror to have more than one measuring areas, and this can provide a control reaction accompanied with the experimental reaction on the chip at the same time. The phase variation of the sample variation with different detecting incident angle can provide more data and can reduce the errors, increase the resolution, and raise the detection ability. To acquire the inference fringes images of the phase, the time-stepped quadrature phase shifting method has been introduced, which required fewer images to retrieve the phase than the five-stepped phase shifting method. The data processing time can be reduced and our system would have the potential to measure the reaction in real-time. Finally, sodium chloride-water solution and Ethanol-water solution in different concentration has been measured to verify our system is workable.

Paper Details

Date Published: 5 May 2015
PDF: 8 pages
Proc. SPIE 9506, Optical Sensors 2015, 95060R (5 May 2015); doi: 10.1117/12.2178697
Show Author Affiliations
Jyun Liao, National Taiwan Ocean Univ. (Taiwan)
Shu-Sheng Lee, National Taiwan Ocean Univ. (Taiwan)
Shih-Yuan Lin, National Taiwan Ocean Univ. (Taiwan)

Published in SPIE Proceedings Vol. 9506:
Optical Sensors 2015
Francesco Baldini; Jiri Homola; Robert A. Lieberman, Editor(s)

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